Effects of nuclear molecules on chromatin function and structure

During the eukaryotic cell cycle and developmental processes, the size of the cell nucleus undergoes dynamic changes. These changes impact not only the DNA concentration within the nucleus but also the physical behavior of molecules in the nucleoplasm. However, it remains an enigma how such alterations in profiles and behaviors of nucleoplasmic molecules can modulate chromatin conformation and function. In this project, we will attempt to develop a cell-free reconstruction system that can selectively manipulate DNA concentration within the nucleus, enabling quantification of the profiles and behaviors of nucleoplasmic molecules and an evaluation of their impact on chromatin function.


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