Understanding genomic modality factors responsible for regulation of transcriptional dynamics

Transcriptional activity of genes is regulated in a cell type-specific manner by the interaction between promoters and distal enhancers. Imaging-based analyses have reported several cases in which there is no clear correlation between enhancer-promoter interactions and transcriptional activity status in individual cells, but the detailed molecular mechanisms are unknown. In this study, in addition to quantifying the nuclear localization and transcriptional activity of specific endogenous genes using a single gene imaging system, we will simultaneously perform live cell imaging of multiple modalities of transcriptional regulators that form clusters in the vicinity of genes upon transcriptional activation and enhancer regions to quantify their nuclear localization. This will clarify the relationship between enhancer-promoter interactions, transcriptional activation and the associated formation of clusters of transcriptional regulators.


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